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Ecotoxicological characterization of waste – Method

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development for determining the ecotoxicological (H14) risk

121

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criterion

1770

LUBW Landesanstalt für Umwelt, Messungen und Naturschutz

2004

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Table of contents

1 Summary.

2 Introduction

3 Principle of bioassays

4 Methodology.

4.1 Sample collection and conservation.

4.2 Sample preparation.

4.3 Selection of the biological test procedures

4.4 Chemical analysis .

4.5 Waste samples

5 Results

5.1 Sampling .

5.2 Sample preparation.

5.3 Eluate preparation.

5.4 Biotest results .

5.4.1 Eluate.

5.4.2 Solid phase .

5.5 Reproducibility and routine suitability of the biotest procedures .

5.5.1 Investigation of the eluate – aquatic test procedure

5.5.2 Investigation of the original sample – tests on solid phase .

5.6 Classification

5.7 Distinguishing between hazardous and non-hazardous waste depending on the biotest result

classification

5.8 Comparison of the classification based on the toxicity classes to the ranking based on the

Preliminary Implementation Manual

5.8.1 Hazardous wastes based on classification as toxicity class 2.

5.8.2 Hazardous wastes based on classification as toxicity class 3.

5.9 Comparison of ecotoxicological and chemical characterization of waste .

5.9.1 Waste group specific description.

5.9.2 Relation between toxicity and chemical parameters

6 Proposed Procedure.

6.1 Definition of a minimum test battery

6.2 Limit Test

6.3 Eluate investigations for genotoxicity with the umu-test

6.4 Economy .

7 Recommendations.

7.1 Eluate investigation .

7.2 Biological test procedures .

8. Literature

9. Appendix.

Appendix I: Orientation values of the Preliminary Implementation Manual (October 2002).

Appendix II: Data sheets - Biological test results and chemical analysis