BEEP PROJECT (Biological Effects of Environmental Pollution in

2,72
MB marine coastal ecosystems)

243
stron

3789
ID LPTC – Université Bordeaux

2005
rok

TABLE OF CONTENT

Participants' information p. 4

Executive Summary of the project p. 7

Work Package 0: Coordination and Management

Task 1: Web site p. 9

Task 2: Project workshops p. 9

Task 3: Preparation of the project reports p. 9

Task 4: Exploitation and dissemination of the results p. 9

Task 5: Administrative and financial management p. 10

Work Package 1: Novel Biomarkers

Task 1: Development of new cellular and molecular biomarkers of stress p. 11

Task 2: Development of new cellular and molecular biomarkers of exposure p. 23

Task 3: Development of a molecular biomarker approach by using protein antibodies and

Mrna probes

p. 33

Task 4: Development of biomarkers able to evaluate pollutant effects on the reproductive

performance of mussels

p. 43

Task 5: Development of biomarkers able to evaluate pollutant effects on the reproductive

performance of fish

p. 54

Task 6: Application of the new developed biomarkers in two common, large, lab

experiments. Application of the new biomarkers on field samples. Comparison of the value

(sensitivity, specificity, etc.) of the new biomarkers with “core biomarkers” used in WP 2 – 3

– 4

p. 60

Work Package 2: Biomonitoring in the Baltic Sea

Task 1:Intercalibration exercises on core biomarkers p. 70

Task 2: Collection of samples and background information on sampling sites p. 72

Task 3: Measurements of biomarkers (core and research) on the target species p. 75

Task 4: Measurements of supporting parameters: chemical contaminants p. 99

Task 5: Data handling p. 100

Work Package 3: Biomonitoring in the Mediterannean Sea

Task 1:Intercalibration exercises on core biomarkers p. 110

Task 2: Collection of samples and background information on sampling sites p. 113

Task 3: Measurements of biomarkers (core and research) on the target species p. 124

Task 4: Measurements of supporting parameters in fish and bivalves: chemical

contaminants and physiological parameters

p. 131

Task 5: Data handling p. 137

Work Package 4: Biomonitoring in the North Atlantic Sea

Task 1:Intercalibration exercises on core biomarkers p. 142

Task 2: site surveys: preparation, collection of samples and background information p. 143

Task 3: Measurements of biomarkers (core and research) on the target species p. 146

Task 4: Measurements of supporting parameters in fish: chemical contaminants and

physiological parameters

p. 154

Task 5: Data handling p. 155

Work Package 5: QA/QC and data management

Task 0:Intercalibration exercises on core biomarkers p. 158

Task 1: Preparation of an expert system p. 158

Task 2: Multivariate analyses of biomarker measurements p. 168

Task 3: Scale classification of pollution level of coastal areas based on multimarker

approach

p. 169

List of publications

p. 182

Multivariate Analysis WP2, WP3, WP4 p. 207